Effect of the coplanarity between camera and leaf for the determination of leaf area in Eichhornia crassipes with digital images
Abstract
The non-destructive methodologies used to determine leaf area through photographic captures and subsequent processing have had an important reception in various disciplines. However, there are few studies that validate the effect of the coplanarity or non-coplanarity between the camera planes and the leaves in terms of precision and accuracy. In this study, the precision of the leaf area values obtained in the Eichhornia crassipes plant was evaluated, using two non-destructive methodologies with image processing in Image J software. Subsequently, the accuracy is evaluated in the non-destructive methodology showing less variability in the results. Method 1 does not present a rigorous control between planes, while methodology 2 uses a device to guarantee coplanarity. As references, an image processing system and printed elliptical figures of known area built with AutoCAD software were used. The results show with a confidence level of 95% the same level of precision between the reference image processing system and methodology 2, which turned out to be the most accurate. Regarding the accuracy, evaluated through the confidence limits for the slope and intercept of curve calibration parameters, a statistically similar behavior was obtained between method 2 and the AutoCAD software, and the same differences between methodology/referents and referent/referrer.
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References
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