Alelos -108C/T, L55M e Q192R de paraoxoase 1 (pon 1) em uma população da região cafeeira colombiana
Resumo
Retomar: paraoxonase 1 (pon 1)é uma enzima integrada nas lipoproteínas de alta densidade (hdl); sua atividade tem sido associada ao efeito antioxidante dessas lipoproteínas. Uma atividade reduzida do mesmo está associada a doenças cardiovasculares.atividade enzimáticaparece depender de seus polimorfismos (C-108T,L55M e Q192R), o que os torna responsáveis pela variabilidade interindividual
e interétnica. Objetivo: caracterizar genotipicamente polimorfismos pon 1 na população mestiça colombiana.
Métodos: sedeterminou as frequências dos alelosRS705379 (C/T-108), RS854560 (L55M) e RS662 (Q192R),do genepor1em 133 adultos saudáveis, com características mestiças, de ambos os sexos e não consanguíneos. A genotipagem foi feita por minisequenciamento (SnaPshot). Resultados: as frequências dos genótipos polimórficos foram:-108 C/T(CC 34%, CT 45%, TT 21%),L55M(LL 50%,
LM 43%, MM 7%) eQ192R(QQ 42%, QR 50%, RR 8%). Seis indivíduos (4,5%) apresentaram haplótipo homozigoto triplo nativo (CC/CC/AA). Conclusão: os resultados indicam que, na população estudada, as frequências genotípicas não foram diferentes das descritas para outras populações latinas e europeias. Além disso, nenhuma ligação forte foi observada entre os três loci estudados.
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