Micropropagation of passion fruit and curuba from nodal and foliar segments.

Abstract

Passionflowers in Colombia are present in 24 departments and 422 municipalities with more than 15000 hectares, represented mainly by small and medium producers. Among the most representative species, passion fruit and curuba stand out. The greatest limitations of the productive sector of these species are conventional propagation techniques, attention to adaptation and climate control of species. Therefore, the objective of this research was to perform in vitro micropropagation of these two species from leaf and nodal segments evaluating different concentrations of Benzyl aminopurine (BAP). The research was developed in three stages: 1) Selection and obtaining of plant material; 2) Evaluation of the disinfection protocol for nodal and leaf segment explants; 3) Standardization of in vitro micropropagation. The disinfection protocol where NaCLO 1.0 and 1.5% was used was the most suitable for the propagation of curuba and passion fruit, however, the 1.0% concentration is the most suitable for controlling contamination by fungi, bacteria and associated factors to necrosis, which favored the development of the explants. The nodal segment was the most suitable for propagation, being more reactive than the foliar segment, under the incidence with BAP. It was found that for obtaining callus, leaves and shoots the best concentration is BAP 1.0 mg/l or the control, while for obtaining leaves BAP 2.0 mg/l. The passion fruit was characterized for being the most outstanding species in the in vitro establishment with respect to the curuba.