Inoculación Experimental de Tres Especies Hospederas de Fitoplasmas en Bogotá, Colombia

  • Laura Margarita Perilla Henao Universidad Militar Nueva Granada
  • Clara Gilma Gutiérrez Castañeda Universidad Militar Nueva Granada
  • Juan José Filgueira Universidad Militar Nueva Granada
  • Liliana Franco-Lara Universidad Militar Nueva Granada
Palabras clave: Fitoplasmas, Ash Y, Apium graveolens, Nicotiana tabacum, Nicotiana debneyi.

Resumen

Los Urapanes (Fraxinus sp.) en Colombia presentan problemas sanitarios como la infección con fitoplasmas del grupo 16SrRNA VII. Para tratar de superar la dificultad que significa trabajar con árboles de gran tamaño, se realizaron ensayos para buscar huéspedes vegetales alternativos que mejoraran la accesibilidad a estos patógenos. En este estudio se hicieron ensayos de inoculación de fitoplasmas de Urapán, permitiendo la visita libre de insectos, sobre cuatro especies vegetales de las familias Solanaceae y Apiaceae como potenciales hospederos alternativos. Una alta proporción de las plantas de Apium graveolens expuestas a insectos (86%) resultó infectada con fitoplasmas, según la sintomatología desarrollada, test DAPI y pruebas moleculares. Una proporción menor de las plantas expuestas de Nicotiana tabacum (50%) y Nicotiana debneyi (10%) presentaron síntomas asociados a infección por fitoplasmas y fueron positiva por test DAPI, mientras que las plantas de Lycopersicum esculentum expuestas no resultaron infectadas. Observaciones mediante el test DAPI mostraron que en las plantas infectadas, los fitoplasmas no presentaron una tendencia marcada a concentrarse en ningún tejido en particular

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Referencias bibliográficas

Barros, T.S.L.; Davis, R.E.; Resende, R.O; Dally, E. L. 2002. Erigeron Witches’-Broom Phytoplasma in Brazil Represents New

Subgroup VII-B in 16S rRNA Gene Group VII, the Ash Yellows Phytoplasma Group. Plant Disease 86, 1142-1148.

Bosco, D; Minucci, C; Boccardo, G. and Conti, M. 1997. Differential acquisition of chrisantemum yellow phytoplasma by three leafhopper species. Entomologia Experimentalis et Applicata 83, 219-224.

Bruni, R; Pellati, F; Bellardi, M.G; Benvenuti,S; Paltrinieri, S; Bertaccini, A. and Bianchi, A. 2005. Herbal Drug Quality and Phytochemical Composition of Hypericum perforatum L. Affected by Ash Yellows Phytoplasma Infection. Journal of Agriculture and Food Chemistry 53, 964 -968.

Bai, X; Fazzolari, T. and Hogenhout, S. 2004. Identification and characterization of TraE genes of Spiroplasma kunkelli. Gene 336, 81-91.

Christensen, N.M; Axelsen, K.B; Nicolaisen, M. and Schulz, A. 2005. Phytoplasmas and their interaction with hosts. Trends in Plant Science 10, 526-535.

Del Serrone, P; Marzachì, C; Cragaloni, M. and Galeffi C. 2001. Phytoplasma infection of tomato in central Italy. Phytopathologia Mediterranea 40, 137–142

Doi, Y; Teranaka, M; Yora, K. and Asuyama H. 1967. Mycoplasma or PLT group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf potato witches’s broom, aster yellows or paulonia witches’ broom. Annual Phytopathology Society Japan 33, 259-266.

Feeley, C.J; Hart, E.R; Thompson, J.R. and Harrington, T.C. 2001. Occurrence, associates symptoms and potential insect vectors of the Ash Yellows phytoplasma in Iowa, U.S. Journal of Arboriculture 27, 331-340.

Filgueira, J.J; Franco-Lara, L; Salcedo, J.E; Gaitán S.L and Boa, E.R. 2004. Urapan (Fraxinus uhdei) dieback: a new disease associated with a phytoplasma in Colombia. New Disease Reports. Plant Pathology 53, 520.

Franco-Lara L. y Filgueira JJ. 2005. Síntomas de decaimiento del Urapán (Fraxinus sp) en Colombia. Fitopatología Colombiana 29, 32-38.

Franco-Lara, L; Contaldo, N; Martinez, C; Calari, A; Paltrinieri, S; Duduk, B. and Bertaccini, A. 2010. Complex phytoplasma infection in declining liquidambar trees in Colombia. Abstract Book 18th Congress International Organization for Mycoplasmology, Chianciano Terme Italy July 2010.

Gaitán, S.L. 2003. Detección y caracterización molecular de ftoplasmas tipo Ash Yellows en Urapanes de Bogotá. Universidad Nacional de Colombia. Postgrado Interfacultades de Microbiología. Tesis de Maestría. Bogotá.

Gundensen, D. E. and Lee, I. M. 1996. Ultrasensitive detection of phytoplasmas by nested PCR assays ussing two universal primer pairs. Phytopathologia Mediterranea. 35, 144-151.

Gundersen D.E; Lee I.-M; Rehner S.A; Davis R.E and Kingsbury D.T. 1994. Phylogeny of mycoplasma-like organisms (phytoplasmas): a basis for their classification. Journal of Bacteriology 176, 5244-5254.

Hearon S.S., Lawson R.H., Smith F.F., McKenzie J.T. and Rosen, J. 1976. Morphology of filamentus forms of a mycoplasmalike organism associated with hydrangea virescence. Phytoplasmology 66, 608-616.

Hibben C.R. and Wolanski, B. 1971. Dodder transmission of a mycoplasma from ash withches’-broom. Phytopathology 61: 151-156.

Hill G.T. and Sinclair, W.A. 2000. Taxa of Leafhoppers Carrying Phytoplasmas at Sites of Ash Yellows Occurrence in New York State. Plant Disease 84, 134-138.

Hogenhout SA, Oshima K, Ammar ED, Kakizawa S, Kingdom HN, Namba, S. 2008. Phytoplasmas: bacteria that manipulate plants and insects. Molecular Plant Pathology 9, 403-423.

Ishiie, T; Doi, Y; Yora, K and Asuyama, H. 1967. Suppresive effects of antibiotics of tetracycline group on symptoms development of mulberry dwarf disease. Annals Phytopathological Society of Japan 33, 267-275.

Jones, P. Phytoplasma Plant Pathogens. In: Plant Pathologist’s Pocketbook. 2002. CABI Publishing. 3rd Ed.

Khan A.J; Botti S; Paltinieri, A.M; Al-Subhi and Bertaccini, A.F.2002. Phytoplasmas in alfalfa seedlings: infected of contaminated seeds? En Abstracts 14 International Organization of Mycoplasmology Conference, Viena, Austria.

Kuske, C.R; Kirkpatric, T.B; Seemuller, E. 1991. Differentiation of virescence MLOs using western aster yellows mycoplasmalike organism chromosomal DNA probes and restriction fragment length polymorphism analysis. Journal of General Microbiology 137, 153-1 59.

Lee, I.M; Davis, R.E; Gundersen, D.E. 2000. Phytoplasma: Phytopathogenic Mollicutes. Annual Review Microbiology, 54: 221-255.

Lee, I.M; Gundersen, D.E. y Bertaccini, A. 1998. Phytoplasma: ecology and genomic diversity. Phytopathology, 88: 1359-1366.

Lee, I.M; Bertaccini, A. Vibio, M. and Gundersen, D.E. 1995. Detection and characterization of phytoplasmas in perennial fruit trees with decline symptoms in Italy. Phytopathology 85, 728-735.

López K. y Filgueira, J.J. Infección y concentración de fitoplasmas tipo AshY en periwinkle (Catharanthus roseus), a partir de árboles de Fraxinus sp. iInfectados. 2006. Revista Facultad de Ciencias Básicas 2, 123-128.

Marcone, C; Neimark, H; Ragozzino, A. and Seemuller, E. 1999. Chromosome sizes of phytoplasmas composing major phylogenetic groups and subgroups. Phytopatology 89, 805-810.

Marcone C, and Seemüller E. 2001. A chromosome map of the European stone fruit yellows phytoplasma. Microbiology 147, 1213-1221

Matteoni, J.A. and Sinclair, W.A. 1988. Elm yellows and Ash yellows. In Tree Mycoplasma Diseases and Epidemiology. Ed. C Hiruki. Pp 19-31. University Alberta Press.

Neimark, H. and Kirkpatrick, B.C. 1993. Isolation and characterization of full-lenght chromosomes from non-culturable plant pathogenic Mycoplasma-like organisms. Molecular Microbiology 7, 21-28.

Orozco, M.L. y Franco-Lara, L. 2005. Ensayos de transmisión de fitoplasmas de Urapan (Fraxinus sp.) con el insecto Tropidosteptes chapingoensis. XXVI Congreso de ASCOLFI Bogotá-Colombia.

Oshima, K; Kakizawa, S; Nishigawa, H; Weiw, W; Susuki, S; Arashida, R; Nakata, D; Miyata, S-I; Ugaki; M Y Namba, S. 2004. Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma. Nature Genetics 36, 27-29.

Pracros, P; Renaudin ,J; Eveillard, S; Mouras, A; Hernould, M. 2006. Tomato Flower Abnormalities Induced by Stolbur Phytoplasma Infection Are Associated with Changes of Expression of Floral Development Genes. Molecular Plant Microbe Interactions 19, 62-68.

Schneider, B; Torres, E; Martin, MP; Schroder, M; Behnke, H.D. and Seemuller, E. 2005. ‘Candidatus Phytoplasma pini’, a novel taxon from Pinus silvestris and Pinus halepensis. International Journal of Systematic and Evolutionary Microbiology 55, 303-307.

Seemüller E. 1976. Investigations to demonstrate mycoplasmalike organisms in diseased plants by fluorescence microscopy. Acta Horticulturae 67, 109–112.

Sinclair, W.A; Luli, R.J; Dyer, A.T. and Larsen, A.O. 1989. Sampling and histological procedures for diagnosis of ash yellows. Plant Disease 73:432-435.

Sinclair, W.A. and Griffiths, H.M. 1994. Ash yellows and its relationship to dieback and decline of Ash. Annual Review of Phytopathology 32, 49-60.

Sinclair, W.A. y Griffiths, H.M. 2000. Variation in aggressiveness of Ash Yellows phytoplasmas. Plant Disease, 84:282-288.

Sinclair WA, Griffiths HM and Davis RE. 1996. Ash Yellows and Lilac Witches’- Broom: phytoplasmal diseases of concern in forestry and horticulture. Plant Disease 80, 468-475.

Sinclair, W.A; Griffiths, H.M. Y Whitlow, T.H. 1997. Comparisons of tolerance of ash yellows phytoplasmas in Fraxinus species and rootstocks-scion combinations. Plant Disease, 81:395-398.

Smart, C.D; Schneider, B; Blomquist, C.L; Guerra, L.J; Harrinson, N.A; Ahrems, U; Lorenz, K.H; Seemüller, E., and Kirkpatrick, B.C. 1996. Phytoplasma-specific PCR iniciadores based on sequences of 16S-23S rRNA spacer region. Applied Environmental Microbiology, 62: 2988-2993.

Tran-Nguyen, L.T.T; Kube, M; Schneider, B; Reinhardt, R. and Gibb, K.S. 2008. Comparative genome analysis of ‘Ca. P.australiense’ (subgroup tuf-Australia I; rp-A) and ‘Ca. Phytoplasma asteris’ strains OY-M and AY-WB. Journal of Bacteriology 190, 3979–3991.

Weintraub, P.G. and Beanland, L. 2006. Insect vectors of phytoplasmas. Annual review of Entomology 51, 91-111

Cómo citar
Perilla Henao, L. M., Gutiérrez Castañeda, C. G., Filgueira, J. J., & Franco-Lara, L. (2016). Inoculación Experimental de Tres Especies Hospederas de Fitoplasmas en Bogotá, Colombia. Revista Facultad De Ciencias Básicas, 6(2), 198–213. https://doi.org/10.18359/rfcb.2074
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2016-09-01
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